PrimePCR™ Probe Assay: ZNF69, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ZNF69, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ZNF69, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055572
Assay Design:   exonic
Chromosome Location:   19:12015520-12015655question
Amplicon Length:   106
Splice Variants Targeted:   ENST00000414255 ENST00000427505 ENST00000457526 ENST00000304060 ENST00000455282 ENST00000304030 ENST00000429654 ENST00000445911 ENST00000340180 ENST00000592625 ENST00000586494 ENST00000343949 ENST00000358987 ENST00000538752 ENST00000545530 ENST00000590798 ENST00000254321 ENST00000482090

Gene Information

Description Not Available

Gene Symbol:   ZNF69
Gene Name:   zinc finger protein 69
Aliases:   Cos5, MGC59928
RefSeq:   NC_000019.9 NT_011295.11
Ensembl:   ENSG00000198429
Entrez:   7620
Chromosome Mapping:   19p13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.050000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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