PrimePCR™ Probe Assay: SLC29A3, Human


Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055555
Assay Design:   exonic
Chromosome Location:   10:73122540-73122677question
Amplicon Length:   108
Splice Variants Targeted:   ENST00000373189

Gene Information

This gene encodes a nucleoside transporter. The encoded protein plays a role in cellular uptake of nucleosides nucleobases and their related analogs. Mutations in this gene have been associated with H syndrome which is characterized by cutaneous hyperpigmentation and hypertrichosis hepatosplenomegaly heart anomalies and hypogonadism. A related disorder PHID (pigmented hypertrichosis with insulin-dependent diabetes mellitus) has also been associated with mutations at this locus. Alternatively spliced transcript variants have been described.[provided by RefSeq Mar 2010]

Gene Symbol:   SLC29A3
Gene Name:   solute carrier family 29 (nucleoside transporters), member 3
Aliases:   ENT3, FLJ11160
RefSeq:   NC_000010.10 NT_030059.13 NG_017066.1
Ensembl:   ENSG00000198246
Entrez:   55315
UniGene:   Hs.438419
Chromosome Mapping:   10q22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.790000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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