This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: DDX42, Human
PrimePCR™ Template for Probe Assay: DDX42, Human
This gene encodes a member of the Asp-Glu-Ala-Asp (DEAD) box protein family. Members of this protein family are putative RNA helicases and are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation nuclear and mitochondrial splicing and ribosome and spliceosome assembly. Members of this family are believed to be involved in embryogenesis spermatogenesis and cellular growth and division. Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.