PrimePCR™ Probe Assay: CACNA1E, Human

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Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055551
Assay Design:   exonic
Chromosome Location:   1:181453015-181479620question
Amplicon Length:   110
Splice Variants Targeted:   ENST00000524607 ENST00000367570 ENST00000526775 ENST00000360108 ENST00000367573 ENST00000357570 ENST00000358338 ENST00000367567

Gene Information

Voltage-dependent calcium channels are multisubunit complexes consisting of alpha-1 alpha-2 beta and delta subunits in a 1:1:1:1 ratio. These channels mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes including muscle contraction hormone or neurotransmitter release gene expression cell motility cell division and cell death. This gene encodes the alpha-1E subunit of the R-type calcium channels which belong to the 'high-voltage activated' group that maybe involved in the modulation of firing patterns of neurons important for information processing. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq Apr 2011]

Gene Symbol:   CACNA1E
Gene Name:   calcium channel, voltage-dependent, R type, alpha 1E subunit
Aliases:   BII, CACH6, CACNL1A6, Cav2.3
RefSeq:   NC_000001.10 NT_004487.19
Ensembl:   ENSG00000198216
Entrez:   777
UniGene:   Hs.437444
Chromosome Mapping:   1q25-q31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.710000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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