PrimePCR™ Probe Assay: HSD17B11, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055549
Assay Design:   exonic
Chromosome Location:   4:88303508-88312128question
Amplicon Length:   93
Splice Variants Targeted:   ENST00000358290 ENST00000507286

Gene Information

Short-chain alcohol dehydrogenases such as HSD17B11 metabolize secondary alcohols and ketones (Brereton et al. 2001 [PubMed 11165019]).[supplied by OMIM Jun 2009]

Gene Symbol:   HSD17B11
Gene Name:   hydroxysteroid (17-beta) dehydrogenase 11
Aliases:   17BHSD11, DHRS8, PAN1B, RETSDR2, SDR16C2
RefSeq:   NC_000004.11 NT_016354.19
Ensembl:   ENSG00000198189
Entrez:   51170
UniGene:   Hs.594923
Chromosome Mapping:   4q22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.830000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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