This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: MIER1, Human
PrimePCR™ Template for Probe Assay: MIER1, Human
This gene encodes a protein that was first identified in Xenopus laevis by its role in a mesoderm induction early response (MIER). In human this gene functions as a growth-factor-induced immediate early gene that encodes a transcriptional regulator. Depending on the cellular context this gene can function as both an activator and repressor of gene transcription. Alternative splicing results in multiple isoforms; some of which contain a nuclear localization signal. [provided by RefSeq Aug 2011]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.