PrimePCR™ Probe Assay: HMGN5, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055543
Assay Design:   exonic
Chromosome Location:   X:80371727-80371824question
Amplicon Length:   68
Splice Variants Targeted:   ENST00000358130 ENST00000447319 ENST00000373250 ENST00000430960 ENST00000436386 ENST00000451455

Gene Information

This gene encodes a nuclear protein with similarities to the high mobility group proteins HMG14 and HMG17 which suggests that this protein may function as a nucleosomal binding and transcriptional activating protein. [provided by RefSeq Sep 2009]

Gene Symbol:   HMGN5
Gene Name:   high mobility group nucleosome binding domain 5
Aliases:   NBP-45, NSBP1
RefSeq:   NC_000023.10 NG_013234.1 NT_011651.17
Ensembl:   ENSG00000198157
Entrez:   79366
UniGene:   Hs.282204
Chromosome Mapping:   Xq13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 36.010000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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