PrimePCR™ Probe Assay: ZNF544, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055540
Assay Design:   exonic
Chromosome Location:   19:58755416-58757749question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000573833 ENST00000224600 ENST00000318003 ENST00000370223 ENST00000370220 ENST00000407006 ENST00000439238 ENST00000338820 ENST00000537645 ENST00000360338 ENST00000599602 ENST00000321545 ENST00000443917 ENST00000598744 ENST00000282286 ENST00000391705 ENST00000299687 ENST00000354670 ENST00000397573 ENST00000220446 ENST00000306376 ENST00000434744 ENST00000367433 ENST00000294725 ENST00000367431 ENST00000599227 ENST00000597240 ENST00000594384 ENST00000596825 ENST00000596652 ENST00000600044 ENST

Gene Information

Description Not Available

Gene Symbol:   ZNF544
Gene Name:   zinc finger protein 544
Aliases:   Not Available
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000198131
Entrez:   27300
UniGene:   Hs.438994
Chromosome Mapping:   19q13.43

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 36.540000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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