PrimePCR™ Probe Assay: KEL, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055521
Assay Design:   exonic
Chromosome Location:   7:142658081-142658461question
Amplicon Length:   96
Splice Variants Targeted:   ENST00000561891 ENST00000562036 ENST00000565337 ENST00000568726 ENST00000355265 ENST00000476829 ENST00000467543 ENST00000460479 ENST00000306320

Gene Information

This gene encodes a type II transmembrane glycoprotein that is the highly polymorphic Kell blood group antigen. The Kell glycoprotein links via a single disulfide bond to the XK membrane protein that carries the Kx antigen. The encoded protein contains sequence and structural similarity to members of the neprilysin (M13) family of zinc endopeptidases. [provided by RefSeq Jul 2008]

Gene Symbol:   KEL
Gene Name:   Kell blood group, metallo-endopeptidase
Aliases:   CD238, ECE3
RefSeq:   NC_000007.13 NG_007492.1 NT_007914.15
Ensembl:   ENSG00000197993
Entrez:   3792
Chromosome Mapping:   7q33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 36.440000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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