PrimePCR™ Probe Assay: ATAD3A, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: ATAD3A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ATAD3A, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055494
Assay Design:   exonic
Chromosome Location:   1:1469822-1469959question
Amplicon Length:   108
Splice Variants Targeted:   ENST00000592696 ENST00000380495 ENST00000558850 ENST00000559185 ENST00000442396 ENST00000262236 ENST00000469391 ENST00000402703 ENST00000561603 ENST00000304330 ENST00000295851 ENST00000424558 ENST00000417864 ENST00000302692 ENST00000339381 ENST00000378756 ENST00000378755 ENST00000339113 ENST00000536055 ENST00000355481 ENST00000359596 ENST00000360985 ENST00000341809 ENST00000537195 ENST00000566029 ENST00000564845 ENST00000398510 ENST00000565803 ENST00000447540 ENST00000252699 ENST00000440400 ENST

Gene Information

ATAD3A and ATAD3B (MIM 612317) are mitochondrial membrane proteins that contribute to the stabilization of large mitochondrial DNA (mtDNA)-protein complexes called nucleoids (He et al. 2007 [PubMed 17210950]).[supplied by OMIM Sep 2008]

Gene Symbol:   ATAD3A
Gene Name:   ATPase family, AAA domain containing 3A
Aliases:   FLJ10709, FLJ35514
RefSeq:   NC_000001.10 NT_004350.19
Ensembl:   ENSG00000197785
Entrez:   55210
Chromosome Mapping:   1p36.33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998000
y-intercept 37.000000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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