PrimePCR™ Probe Assay: SERPINB2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055479
Assay Design:   exonic
Chromosome Location:   18:61562534-61564335question
Amplicon Length:   65
Splice Variants Targeted:   ENST00000404622 ENST00000299502 ENST00000413956 ENST00000443281 ENST00000457692

Gene Information

Description Not Available

Gene Symbol:   SERPINB2
Gene Name:   serpin peptidase inhibitor, clade B (ovalbumin), member 2
Aliases:   HsT1201, PAI, PAI-2, PAI2, PLANH2
RefSeq:   NC_000018.9 NT_025028.14
Ensembl:   ENSG00000197632
Entrez:   5055
UniGene:   Hs.594481
Chromosome Mapping:   18q21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998500
y-intercept 35.310000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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