PrimePCR™ Probe Assay: VEPH1, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055454
Assay Design:   exonic
Chromosome Location:   3:157213051-157213198question
Amplicon Length:   118
Splice Variants Targeted:   ENST00000392833 ENST00000362010 ENST00000392832 ENST00000494677 ENST00000468233 ENST00000487753 ENST00000461299 ENST00000489602 ENST00000543418 ENST00000537559

Gene Information

Description Not Available

Gene Symbol:   VEPH1
Gene Name:   ventricular zone expressed PH domain homolog 1 (zebrafish)
Aliases:   FLJ12604, KIAA1692, MELT, MGC111426, MGC126709, MGC142115, VEPH
RefSeq:   NC_000003.11 NT_005612.16
Ensembl:   ENSG00000197415
Entrez:   79674
UniGene:   Hs.658046
Chromosome Mapping:   3q24-q25

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.590000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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