PrimePCR™ Probe Assay: SLC22A5, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055447
Assay Design:   exonic
Chromosome Location:   5:131719846-131719938question
Amplicon Length:   63
Splice Variants Targeted:   ENST00000245407 ENST00000415928 ENST00000435065

Gene Information

Polyspecific organic cation transporters in the liver kidney intestine and other organs are critical for elimination of many endogenous small organic cations as well as a wide array of drugs and environmental toxins. The encoded protein is a plasma integral membrane protein which functions both as an organic cation transporter and as a sodium-dependent high affinity carnitine transporter. The encoded protein is involved in the active cellular uptake of carnitine. Mutations in this gene are the cause of systemic primary carnitine deficiency (CDSP) an autosomal recessive disorder manifested early in life by hypoketotic hypoglycemia and acute metabolic decompensation and later in life by skeletal myopathy or cardiomyopathy. [provided by RefSeq Jul 2008]

Gene Symbol:   SLC22A5
Gene Name:   solute carrier family 22 (organic cation/carnitine transporter), member 5
Aliases:   CDSP, FLJ46769, OCTN2, OCTN2VT
RefSeq:   NC_000005.9 NG_008982.1 NT_034772.6
Ensembl:   ENSG00000197375
Entrez:   6584
UniGene:   Hs.443572
Chromosome Mapping:   5q31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.950000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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