PrimePCR™ Probe Assay: ZNF836, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055318
Assay Design:   exonic
Chromosome Location:   19:52660681-52663724question
Amplicon Length:   90
Splice Variants Targeted:   ENST00000594295 ENST00000426391 ENST00000389534 ENST00000600228 ENST00000330123 ENST00000596290 ENST00000597252 ENST00000596428 ENST00000322146 ENST00000600714 ENST00000597597 ENST00000541777 ENST00000545872 ENST00000596930 ENST00000597161 ENST00000598536 ENST00000544146 ENST00000536937 ENST00000301096

Gene Information

Description Not Available

Gene Symbol:   ZNF836
Gene Name:   zinc finger protein 836
Aliases:   FLJ16287
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000196267
Entrez:   162962
UniGene:   Hs.631584
Chromosome Mapping:   19q13.41

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.580000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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