PrimePCR™ Probe Assay: PLEKHG4, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: PLEKHG4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PLEKHG4, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055306
Assay Design:   exonic
Chromosome Location:   16:67313859-67314056question
Amplicon Length:   168
Splice Variants Targeted:   ENST00000360461 ENST00000562144 ENST00000565899 ENST00000565773 ENST00000562744 ENST00000427155 ENST00000379344 ENST00000568621 ENST00000450733 ENST00000567938

Gene Information

The protein encoded by this gene contains multiple domains suggestive of a role in intracellular signaling and cytoskeleton dynamics at the Golgi apparatus. Mutations in this gene are associated with spinocerebellar ataxia 16q22-linked. Several alternatively spliced transcript variants differing only in the 5' UTR or encoding a different isoform have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   PLEKHG4
Gene Name:   pleckstrin homology domain containing, family G (with RhoGef domain) member 4
Aliases:   ARHGEF44, DKFZp434I216, PRTPHN1, SCA4
RefSeq:   NC_000016.9 NT_010498.15 NG_008439.1
Ensembl:   ENSG00000196155
Entrez:   25894
Chromosome Mapping:   16q22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 36.140000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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