PrimePCR™ Probe Assay: PLEKHG4, Human


Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055306
Assay Design:   exonic
Chromosome Location:   16:67313859-67314056question
Amplicon Length:   168
Splice Variants Targeted:   ENST00000360461 ENST00000562144 ENST00000565899 ENST00000565773 ENST00000562744 ENST00000427155 ENST00000379344 ENST00000568621 ENST00000450733 ENST00000567938

Gene Information

The protein encoded by this gene contains multiple domains suggestive of a role in intracellular signaling and cytoskeleton dynamics at the Golgi apparatus. Mutations in this gene are associated with spinocerebellar ataxia 16q22-linked. Several alternatively spliced transcript variants differing only in the 5' UTR or encoding a different isoform have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   PLEKHG4
Gene Name:   pleckstrin homology domain containing, family G (with RhoGef domain) member 4
Aliases:   ARHGEF44, DKFZp434I216, PRTPHN1, SCA4
RefSeq:   NC_000016.9 NT_010498.15 NG_008439.1
Ensembl:   ENSG00000196155
Entrez:   25894
UniGene:   Hs.188781
Chromosome Mapping:   16q22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 36.140000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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