PrimePCR™ Probe Assay: BLOC1S2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: BLOC1S2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: BLOC1S2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055297
Assay Design:   exonic
Chromosome Location:   10:102035240-102039976question
Amplicon Length:   77
Splice Variants Targeted:   ENST00000441611 ENST00000370372 ENST00000579542

Gene Information

This gene encodes a protein with multiple functions. The encoded protein has been found in association with the centrosome shown to co-localize with gamma-tubulin and also found to be one of the proteins in the BLOC-1 complex which functions in the formation of lysosome-related organelles. A pseudogene of this gene is located on the X chromosome. Alternative splicing results in multiple transcript variants. [provided by RefSeq Feb 2012]

Gene Symbol:   BLOC1S2
Gene Name:   biogenesis of lysosomal organelles complex-1, subunit 2
Aliases:   BLOS2, FLJ30135, MGC10120, RP11-316M21.4
RefSeq:   NC_000010.10 NT_030059.13
Ensembl:   ENSG00000196072
Entrez:   282991
Chromosome Mapping:   10q24.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.580000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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