PrimePCR™ Probe Assay: KCTD21, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055272
Assay Design:   exonic
Chromosome Location:   11:77885488-77885577question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000340067 ENST00000526208 ENST00000525447 ENST00000529350 ENST00000530018 ENST00000528776 ENST00000338754 ENST00000353479 ENST00000369733 ENST00000340001 ENST00000453985 ENST00000370732 ENST00000409671 ENST00000566264 ENST00000369135 ENST00000460099 ENST00000352832 ENST00000425826 ENST00000366552 ENST00000581312

Gene Information

Description Not Available

Gene Symbol:   KCTD21
Gene Name:   potassium channel tetramerisation domain containing 21
Aliases:   Not Available
RefSeq:   NC_000011.9 NT_167190.1
Ensembl:   ENSG00000188997
Entrez:   283219
UniGene:   Hs.528439
Chromosome Mapping:   11q14.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 36.370000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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