PrimePCR™ Probe Assay: TRMT2B, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055266
Assay Design:   exonic
Chromosome Location:   X:100274052-100274392question
Amplicon Length:   98
Splice Variants Targeted:   ENST00000372939 ENST00000372935 ENST00000372936 ENST00000338687 ENST00000545398 ENST00000372931

Gene Information

This gene encodes a homolog of the TRM2 gene in S. cerevisiae. The yeast gene encodes a tRNA methyltransferase that plays a role in tRNA maturation. The yeast protein also has endo-exonuclease activity and may be involved in DNA double strand break repair. Alternative splicing results in multiple transcripts encoding different isoforms. [provided by RefSeq Nov 2009]

Gene Symbol:   TRMT2B
Gene Name:   TRM2 tRNA methyltransferase 2 homolog B (S. cerevisiae)
Aliases:   CXorf34, FLJ12687, dJ341D10.3
RefSeq:   NC_000023.10 NT_011651.17 NG_015971.1
Ensembl:   ENSG00000188917
Entrez:   79979
UniGene:   Hs.496501
Chromosome Mapping:   Xq22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.660000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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