PrimePCR™ Probe Assay: SNTN, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055261
Assay Design:   exonic
Chromosome Location:   3:63638359-63638458question
Amplicon Length:   70
Splice Variants Targeted:   ENST00000374572 ENST00000552779 ENST00000550330 ENST00000383198 ENST00000550915 ENST00000549142 ENST00000379445 ENST00000395322 ENST00000343837 ENST00000469440 ENST00000496807 ENST00000434618 ENST00000456592 ENST00000417059 ENST00000549949 ENST00000553235 ENST00000383066 ENST00000551943 ENST00000546700 ENST00000249330 ENST00000445482 ENST00000348628 ENST00000533760 ENST00000401611 ENST00000316851 ENST00000416701 ENST00000591454 ENST00000593059 ENST00000591218 ENST00000590031 ENST00000576604 ENST

Gene Information

Description Not Available

Gene Symbol:   SNTN
Gene Name:   sentan, cilia apical structure protein
Aliases:   FLJ44379, S100A1L, S100AL, sentan
RefSeq:   NC_000003.11 NT_022517.18
Ensembl:   ENSG00000188817
Entrez:   132203
UniGene:   Hs.130704
Chromosome Mapping:   3p14.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.580000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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