PrimePCR™ Probe Assay: CATSPER4, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055257
Assay Design:   exonic
Chromosome Location:   1:26524889-26526488question
Amplicon Length:   106
Splice Variants Targeted:   ENST00000338855 ENST00000456354

Gene Information

Description Not Available

Gene Symbol:   CATSPER4
Gene Name:   cation channel, sperm associated 4
Aliases:   MGC149223
RefSeq:   NC_000001.10 NT_004610.19
Ensembl:   ENSG00000188782
Entrez:   378807
UniGene:   Hs.123532
Chromosome Mapping:   1p35.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.994600
y-intercept 37.350000
Efficiency 92

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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