PrimePCR™ Probe Assay: SECISBP2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: SECISBP2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SECISBP2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055184
Assay Design:   exonic
Chromosome Location:   9:91956264-91956389question
Amplicon Length:   96
Splice Variants Targeted:   ENST00000358273 ENST00000356175 ENST00000217939 ENST00000358763 ENST00000332840 ENST00000375807 ENST00000339901 ENST00000425851 ENST00000534113

Gene Information

The incorporation of selenocysteine into a protein requires the concerted action of an mRNA element called a sec insertion sequence (SECIS) a selenocysteine-specific translation elongation factor and a SECIS binding protein. With these elements in place a UGA codon can be decoded as selenocysteine. The gene described in this record encodes a nuclear protein that functions as a SECIS binding protein. Mutations in this gene have been associated with a reduction in activity of a specific thyroxine deiodinase a selenocysteine-containing enzyme and abnormal thyroid hormone metabolism. [provided by RefSeq Jul 2008]

Gene Symbol:   SECISBP2
Gene Name:   SECIS binding protein 2
Aliases:   DKFZp686C09169, SBP2
RefSeq:   NC_000009.11 NG_012177.1 NT_008470.19
Ensembl:   ENSG00000187742
Entrez:   79048
Chromosome Mapping:   9q22.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.100000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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