PrimePCR™ Probe Assay: AMTN, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: AMTN, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: AMTN, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055181
Assay Design:   exonic
Chromosome Location:   4:71396926-71398195question
Amplicon Length:   86
Splice Variants Targeted:   ENST00000572084 ENST00000572361 ENST00000571646 ENST00000572660 ENST00000339336 ENST00000504451 ENST00000404233 ENST00000529972 ENST00000525859 ENST00000530473 ENST00000543287

Gene Information

The mineralized portions of teeth the dentin and enamel are formed by mesenchyme-derived odontoblasts and epithelium-derived ameloblasts respectively. As ameloblasts differentiate they deposit specific proteins necessary for enamel formation including amelogenin (AMELX; MIM 300391) enamelin (ENAM; MIM 606585) and ameloblastin (AMBN; MIM 601259) in the organic enamel matrix. Amelotin is specifically expressed in maturation-stage ameloblasts (Iwasaki et al. 2005 [PubMed 16304441]).[supplied by OMIM Mar 2008]

Gene Symbol:   AMTN
Gene Name:   amelotin
Aliases:   MGC148132, MGC148133, UNQ689
RefSeq:   NC_000004.11 NT_022778.16
Ensembl:   ENSG00000187689
Entrez:   401138
Chromosome Mapping:   4q13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.750000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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