PrimePCR™ Probe Assay: FNBP1, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055159
Assay Design:   exonic
Chromosome Location:   9:132652819-132658234question
Amplicon Length:   95
Splice Variants Targeted:   ENST00000446176 ENST00000449089 ENST00000355681 ENST00000420781 ENST00000443566

Gene Information

The protein encoded by this gene is a member of the formin-binding-protein family. The protein contains an N-terminal Fer/Cdc42-interacting protein 4 (CIP4) homology (FCH) domain followed by a coiled-coil domain a proline-rich motif a second coiled-coil domain a Rho family protein-binding domain (RBD) and a C-terminal SH3 domain. This protein binds sorting nexin 2 (SNX2) tankyrase (TNKS) and dynamin; an interaction between this protein and formin has not been demonstrated yet in human. [provided by RefSeq Jul 2008]

Gene Symbol:   FNBP1
Gene Name:   formin binding protein 1
Aliases:   FBP17, KIAA0554, MGC126804
RefSeq:   NC_000009.11 NT_008470.19
Ensembl:   ENSG00000187239
Entrez:   23048
UniGene:   Hs.189409
Chromosome Mapping:   9q34

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 36.490000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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