PrimePCR™ Probe Assay: PNLIPRP1, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055143
Assay Design:   exonic
Chromosome Location:   10:118351295-118351930question
Amplicon Length:   116
Splice Variants Targeted:   ENST00000574116 ENST00000571034 ENST00000572871 ENST00000572502 ENST00000572926 ENST00000572596 ENST00000576132 ENST00000398587 ENST00000518882 ENST00000531984 ENST00000358834 ENST00000528052 ENST00000530319 ENST00000527980 ENST00000471549 ENST00000534537 ENST00000442761 ENST00000335551 ENST00000400186 ENST00000602723 ENST00000520002 ENST00000602557 ENST00000537824 ENST00000542608 ENST00000539096

Gene Information

Description Not Available

Gene Symbol:   PNLIPRP1
Gene Name:   pancreatic lipase-related protein 1
Aliases:   PLRP1
RefSeq:   NC_000010.10 NT_030059.13
Ensembl:   ENSG00000187021
Entrez:   5407
UniGene:   Hs.73923
Chromosome Mapping:   10q25.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997200
y-intercept 38.610000
Efficiency 92

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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