PrimePCR™ Probe Assay: ESPN, Human


Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055142
Assay Design:   exonic
Chromosome Location:   1:6517314-6520130question
Amplicon Length:   63
Splice Variants Targeted:   ENST00000529997 ENST00000377828 ENST00000461727 ENST00000434576 ENST00000416731 ENST00000303965 ENST00000359246 ENST00000375376

Gene Information

This gene encodes a multifunctional actin-bundling protein. It plays a major role in regulating the organization dimensions dynamics and signaling capacities of the actin filament-rich microvillus-type specializations that mediate sensory transduction in various mechanosensory and chemosensory cells. Mutations in this gene are associated with autosomal recessive neurosensory deafness and autosomal dominant sensorineural deafness without vestibular involvement. [provided by RefSeq Nov 2009]

Gene Symbol:   ESPN
Gene Name:   espin
Aliases:   DFNB36, DKFZp434A196, DKFZp434G2126
RefSeq:   NC_000001.10 NG_015866.1 NT_021937.19
Ensembl:   ENSG00000187017
Entrez:   83715
UniGene:   Hs.732470
Chromosome Mapping:   1p36.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997200
y-intercept 36.950000
Efficiency 105

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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