This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: MAPT, Human
PrimePCR™ Template for Probe Assay: MAPT, Human
This gene encodes the microtubule-associated protein tau (MAPT) whose transcript undergoes complex regulated alternative splicing giving rise to several mRNA species. MAPT transcripts are differentially expressed in the nervous system depending on stage of neuronal maturation and neuron type. MAPT gene mutations have been associated with several neurodegenerative disorders such as Alzheimer's disease Pick's disease frontotemporal dementia cortico-basal degeneration and progressive supranuclear palsy. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.