PrimePCR™ Probe Assay: HTR3E, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055054
Assay Design:   exonic
Chromosome Location:   3:183822643-183823069question
Amplicon Length:   87
Splice Variants Targeted:   ENST00000415389 ENST00000425359 ENST00000335304 ENST00000431041 ENST00000436361 ENST00000440596

Gene Information

This locus encodes a 5-hydroxytryptamine (serotonin) receptor subunit. The encoded protein subunit E may play a role in neurotransmission in myenteric neurons. Genes encoding subunits C D and E form a cluster on chromosome 3. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq Feb 2012]

Gene Symbol:   HTR3E
Gene Name:   5-hydroxytryptamine (serotonin) receptor 3, family member E
Aliases:   5-HT3c1, MGC120035, MGC120036, MGC120037
RefSeq:   NC_000003.11 NG_012452.1 NT_005612.16
Ensembl:   ENSG00000186038
Entrez:   285242
UniGene:   Hs.449179
Chromosome Mapping:   3q27.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.970000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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