This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: DNAH14, Human
PrimePCR™ Template for Probe Assay: DNAH14, Human
Dyneins are microtubule-associated motor protein complexes composed of several heavy light and intermediate chains. Two major classes of dyneins axonemal and cytoplasmic have been identified. DNAH14 is an axonemal dynein heavy chain (DHC) (Vaughan et al. 1996 [PubMed 8812413]).[supplied by OMIM Mar 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.