PrimePCR™ Probe Assay: LSAMP, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: LSAMP, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: LSAMP, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055015
Assay Design:   exonic
Chromosome Location:   3:115805266-115805390question
Amplicon Length:   95
Splice Variants Targeted:   ENST00000333617 ENST00000490035 ENST00000474851 ENST00000539563

Gene Information

The protein encoded by this gene is a neuronal surface glycoprotein found in cortical and subcortical regions of the limbic system. During development of the limbic system this encoded protein is found on the surface of axonal membranes and growth cones where it acts as a selective homophilic adhesion molecule and guides the development of specific patterns of neuronal connections. [provided by RefSeq Jul 2008]

Gene Symbol:   LSAMP
Gene Name:   limbic system-associated membrane protein
Aliases:   FLJ34254, FLJ35396, FLJ37216, FLJ54658, IGLON3, LAMP
RefSeq:   NC_000003.11 NT_005612.16
Ensembl:   ENSG00000185565
Entrez:   4045
Chromosome Mapping:   3q13.2-q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 1.000000
y-intercept 35.950000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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