PrimePCR™ Probe Assay: BRCC3, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055013
Assay Design:   exonic
Chromosome Location:   X:154305445-154305549question
Amplicon Length:   75
Splice Variants Targeted:   ENST00000340647 ENST00000330045 ENST00000369459 ENST00000369462 ENST00000411985 ENST00000399026 ENST00000399042 ENST00000597217 ENST00000601378 ENST00000597826 ENST00000594541 ENST00000596060 ENST00000597317 ENST00000600398

Gene Information

This gene encodes a subunit of the BRCA1-BRCA2-containing complex (BRCC) which is an E3 ubiquitin ligase. This complex plays a role in the DNA damage response where it is responsible for the stable accumulation of BRCA1 at DNA break sites. The component encoded by this gene can specifically cleave Lys 63-linked polyubiquitin chains and it regulates the abundance of these polyubiquitin chains in chromatin. The loss of this gene results in abnormal angiogenesis and is associated with syndromic moyamoya a cerebrovascular angiopathy. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 5. [provided by RefSeq Jun 2011]

Gene Symbol:   BRCC3
Gene Name:   BRCA1/BRCA2-containing complex, subunit 3
Aliases:   BRCC36, C6.1A, CXorf53
RefSeq:   NC_000023.10 NG_005114.1 NT_167198.1
Ensembl:   ENSG00000185515
Entrez:   79184
UniGene:   Hs.558537
Chromosome Mapping:   Xq28

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 36.300000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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