PrimePCR™ Probe Assay: PRMT3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0054991
Assay Design:   exonic
Chromosome Location:   11:20530026-20530136question
Amplicon Length:   81
Splice Variants Targeted:   ENST00000331079 ENST00000437750

Gene Information

Type I protein arginine N-methyltransferases (PRMTs) such as PRMT3 catalyze the formation of asymmetric N(G)N(G)-dimethylarginine (ADMA) residues in proteins (Tang et al. 1998 [PubMed 9642256]).[supplied by OMIM Mar 2008]

Gene Symbol:   PRMT3
Gene Name:   protein arginine methyltransferase 3
Aliases:   HRMT1L3
RefSeq:   NC_000011.9 NT_009237.18
Ensembl:   ENSG00000185238
Entrez:   10196
Chromosome Mapping:   11p15.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.390000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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