PrimePCR™ Probe Assay: NT5C1B, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0054971
Assay Design:   exonic
Chromosome Location:   2:18745152-18745297question
Amplicon Length:   116
Splice Variants Targeted:   ENST00000594436 ENST00000597784 ENST00000263369 ENST00000597140 ENST00000532967 ENST00000304081 ENST00000359846 ENST00000418427 ENST00000600945

Gene Information

Cytosolic 5-prime nucleotidases such as NT5C1B catalyze production of adenosine which regulates diverse physiologic processes (Sala-Newby and Newby 2001 [PubMed 11690631]).[supplied by OMIM Mar 2008]

Gene Symbol:   NT5C1B
Gene Name:   5'-nucleotidase, cytosolic IB
Aliases:   AIRP, CN-IB, CN1B, MGC26640
RefSeq:   NC_000002.11 NT_015926.15
Ensembl:   ENSG00000185013
Entrez:   93034
UniGene:   Hs.120319
Chromosome Mapping:   2p24.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 36.820000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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