PrimePCR™ Probe Assay: ARSI, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0054896
Assay Design:   exonic
Chromosome Location:   5:149677906-149678036question
Amplicon Length:   101
Splice Variants Targeted:   ENST00000328668 ENST00000515301 ENST00000509146

Gene Information

Sulfatases (EC 3.1.5.6) such as ARSI hydrolyze sulfate esters from sulfated steroids carbohydrates proteoglycans and glycolipids. They are involved in hormone biosynthesis modulation of cell signaling and degradation of macromolecules (Sardiello et al. 2005 [PubMed 16174644]).[supplied by OMIM Mar 2008]

Gene Symbol:   ARSI
Gene Name:   arylsulfatase family, member I
Aliases:   FLJ16069
RefSeq:   NC_000005.9 NT_029289.11
Ensembl:   ENSG00000183876
Entrez:   340075
UniGene:   Hs.591252
Chromosome Mapping:   5q32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 36.770000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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