PrimePCR™ Probe Assay: FHL3, Human

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Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0054851
Assay Design:   exonic
Chromosome Location:   1:38464714-38464931question
Amplicon Length:   80
Splice Variants Targeted:   ENST00000373016

Gene Information

The protein encoded by this gene is a member of a family of proteins containing a four-and-a-half LIM domain which is a highly conserved double zinc finger motif. The encoded protein has been shown to interact with the cancer developmental regulators SMAD2 SMAD3 and SMAD4 the skeletal muscle myogenesis protein MyoD and the high-affinity IgE beta chain regulator MZF-1. This protein may be involved in tumor suppression repression of MyoD expression and repression of IgE receptor expression. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Aug 2011]

Gene Symbol:   FHL3
Gene Name:   four and a half LIM domains 3
Aliases:   MGC19547, MGC23614, MGC8696, SLIM2
RefSeq:   NC_000001.10 NT_032977.9
Ensembl:   ENSG00000183386
Entrez:   2275
UniGene:   Hs.57687
Chromosome Mapping:   1p34

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.860000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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