This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: RGS6, Human
PrimePCR™ Template for Probe Assay: RGS6, Human
This gene encodes a member of the RGS (regulator of G protein signaling) family of proteins which are defined by the presence of a RGS domain that confers the GTPase-activating activity of these proteins toward certain G alpha subunits. This protein also belongs to a subfamily of RGS proteins characterized by the presence of DEP and GGL domains the latter a G beta 5-interacting domain. The RGS proteins negatively regulate G protein signaling and may modulate neuronal cardiovascular lymphocytic activities and cancer risk. Many alternatively spliced transcript variants encoding different isoforms with long or short N-terminal domains complete or incomplete GGL domains and distinct C-terminal domains have been described for this gene however the full-length nature of some of these variants is not known.[provided by RefSeq Mar 2011]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.