PrimePCR™ Probe Assay: ZNF716, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: ZNF716, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ZNF716, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0054769
Assay Design:   exonic
Chromosome Location:   7:57522847-57528539question
Amplicon Length:   108
Splice Variants Targeted:   ENST00000570211 ENST00000419098 ENST00000512994 ENST00000240499 ENST00000331162 ENST00000420713 ENST00000421025 ENST00000255746 ENST00000309683 ENST00000328747 ENST00000431504 ENST00000450302 ENST00000357512 ENST00000261560 ENST00000397126 ENST00000397121 ENST00000594710 ENST00000599851 ENST00000402377 ENST00000528059 ENST00000531570 ENST00000395385 ENST00000577063 ENST00000569383

Gene Information

Description Not Available

Gene Symbol:   ZNF716
Gene Name:   zinc finger protein 716
Aliases:   FLJ46189
RefSeq:   NC_000007.13 NT_033968.6
Ensembl:   ENSG00000182111
Entrez:   441234
Chromosome Mapping:   7p11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.740000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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