PrimePCR™ Probe Assay: S1PR5, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: S1PR5, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: S1PR5, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0054689
Assay Design:   exonic
Chromosome Location:   19:10624159-10624279question
Amplicon Length:   91
Splice Variants Targeted:   ENST00000333430 ENST00000439028

Gene Information

The lysosphingolipid sphingosine 1-phosphate (S1P) regulates cell proliferation apoptosis motility and neurite retraction. Its actions may be both intracellular as a second messenger and extracellular as a receptor ligand. S1P and the structurally related lysolipid mediator lysophosphatidic acid (LPA) signal cells through a set of G protein-coupled receptors known as EDG receptors. Some EDG receptors (e.g. EDG1; MIM 601974) are S1P receptors; others (e.g. EDG2; MIM 602282) are LPA receptors.[supplied by OMIM Mar 2008]

Gene Symbol:   S1PR5
Gene Name:   sphingosine-1-phosphate receptor 5
Aliases:   EDG8, Edg-8, S1P5, SPPR-1, SPPR-2
RefSeq:   NC_000019.9 NT_011295.11
Ensembl:   ENSG00000180739
Entrez:   53637
Chromosome Mapping:   19p13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.890000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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