PrimePCR™ Probe Assay: ZNF771, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0054657
Assay Design:   exonic
Chromosome Location:   16:30419386-30419498question
Amplicon Length:   83
Splice Variants Targeted:   ENST00000274712 ENST00000455860 ENST00000436311 ENST00000399344 ENST00000383194 ENST00000319296 ENST00000566625 ENST00000564550 ENST00000434417 ENST00000251413 ENST00000369044 ENST00000344683 ENST00000519480 ENST00000567107 ENST00000312015 ENST00000345070 ENST00000396879 ENST00000299989 ENST00000381761 ENST00000321596 ENST00000354127 ENST00000266000 ENST00000374542 ENST00000414083 ENST00000399060 ENST00000383062 ENST00000405315 ENST00000407032 ENST00000263273 ENST00000370904 ENST00000433482 ENST

Gene Information

Description Not Available

Gene Symbol:   ZNF771
Gene Name:   zinc finger protein 771
Aliases:   DSC43
RefSeq:   NC_000016.9 NT_010393.16
Ensembl:   ENSG00000179965
Entrez:   51333
UniGene:   Hs.148584
Chromosome Mapping:   16p11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.740000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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