PrimePCR™ Probe Assay: CDC42EP4, Human

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Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0054633
Assay Design:   exonic
Chromosome Location:   17:71282617-71282748question
Amplicon Length:   102
Splice Variants Targeted:   ENST00000579611 ENST00000580315 ENST00000581014 ENST00000439510 ENST00000335793 ENST00000415525 ENST00000418465 ENST00000427477 ENST00000400325

Gene Information

The product of this gene is a member of the CDC42-binding protein family. Members of this family interact with Rho family GTPases and regulate the organization of the actin cytoskeleton. This protein has been shown to bind both CDC42 and TC10 GTPases in a GTP-dependent manner. When overexpressed in fibroblasts this protein was able to induce pseudopodia formation which suggested a role in inducing actin filament assembly and cell shape control. [provided by RefSeq Jul 2008]

Gene Symbol:   CDC42EP4
Gene Name:   CDC42 effector protein (Rho GTPase binding) 4
Aliases:   BORG4, CEP4, KAIA1777, MGC17125, MGC3740
RefSeq:   NC_000017.10 NT_010783.15
Ensembl:   ENSG00000179604
Entrez:   23580
UniGene:   Hs.3903
Chromosome Mapping:   17q24-q25

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.940000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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