PrimePCR™ Probe Assay: HNRNPCL1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: HNRNPCL1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: HNRNPCL1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0054613
Assay Design:   exonic
Chromosome Location:   1:12907667-12907819question
Amplicon Length:   123
Splice Variants Targeted:   ENST00000336053 ENST00000554969 ENST00000556142 ENST00000554455 ENST00000430246 ENST00000553753 ENST00000555914 ENST00000555309 ENST00000556628 ENST00000556513 ENST00000557201 ENST00000553300 ENST00000556897 ENST00000420743 ENST00000557157 ENST00000554539 ENST00000554383 ENST00000555215 ENST00000555137 ENST00000320084 ENST00000449098 ENST00000317869

Gene Information

Description Not Available

Gene Symbol:   HNRNPCL1
Gene Name:   heterogeneous nuclear ribonucleoprotein C-like 1
Aliases:   HNRPCL1
RefSeq:   NC_000001.10 NT_021937.19
Ensembl:   ENSG00000179172
Entrez:   343069
Chromosome Mapping:   1p36.21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.320000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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