This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: GLDC, Human
PrimePCR™ Template for Probe Assay: GLDC, Human
Degradation of glycine is brought about by the glycine cleavage system which is composed of four mitochondrial protein components: P protein (a pyridoxal phosphate-dependent glycine decarboxylase) H protein (a lipoic acid-containing protein) T protein (a tetrahydrofolate-requiring enzyme) and L protein (a lipoamide dehydrogenase). The protein encoded by this gene is the P protein which binds to glycine and enables the methylamine group from glycine to be transferred to the T protein. Defects in this gene are a cause of nonketotic hyperglycinemia (NKH).[provided by RefSeq Jan 2010]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.