PrimePCR™ Probe Assay: CALML5, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CALML5, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CALML5, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0054567
Assay Design:   exonic
Chromosome Location:   10:5541179-5541310question
Amplicon Length:   102
Splice Variants Targeted:   ENST00000264012 ENST00000581171 ENST00000378625 ENST00000344463 ENST00000557004 ENST00000555095 ENST00000298841 ENST00000293396 ENST00000394709 ENST00000247207 ENST00000380332 ENST00000392607 ENST00000330175 ENST00000285206 ENST00000320521

Gene Information

This gene encodes a novel calcium binding protein expressed in the epidermis and related to the calmodulin family of calcium binding proteins. Functional studies with recombinant protein demonstrate it does bind calcium and undergoes a conformational change when it does so. Abundant expression is detected only in reconstructed epidermis and is restricted to differentiating keratinocytes. In addition it can associate with transglutaminase 3 shown to be a key enzyme in the terminal differentiation of keratinocytes. [provided by RefSeq Jul 2008]

Gene Symbol:   CALML5
Gene Name:   calmodulin-like 5
Aliases:   CLSP
RefSeq:   NC_000010.10 NT_008705.16
Ensembl:   ENSG00000178372
Entrez:   51806
Chromosome Mapping:   10p15.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.910000
Efficiency 105

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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