PrimePCR™ Probe Assay: IMPDH2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0054544
Assay Design:   exonic
Chromosome Location:   3:49064238-49064409question
Amplicon Length:   69
Splice Variants Targeted:   ENST00000429182 ENST00000326739 ENST00000442157

Gene Information

This gene encodes the rate-limiting enzyme in the de novo guanine nucleotide biosynthesis. It is thus involved in maintaining cellular guanine deoxy- and ribonucleotide pools needed for DNA and RNA synthesis. The encoded protein catalyzes the NAD-dependent oxidation of inosine-5'-monophosphate into xanthine-5'-monophosphate which is then converted into guanosine-5'-monophosphate. This gene is up-regulated in some neoplasms suggesting it may play a role in malignant transformation. [provided by RefSeq Jul 2008]

Gene Symbol:   IMPDH2
Gene Name:   IMP (inosine 5'-monophosphate) dehydrogenase 2
Aliases:   IMPD2, IMPDH-II
RefSeq:   NC_000003.11 NG_012091.1 NG_016282.1 NT_022517.18
Ensembl:   ENSG00000178035
Entrez:   3615
UniGene:   Hs.654400
Chromosome Mapping:   3p21.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.140000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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