PrimePCR™ Probe Assay: SRRM3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: SRRM3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SRRM3, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0054520
Assay Design:   exonic
Chromosome Location:   7:75915427-75915543question
Amplicon Length:   87
Splice Variants Targeted:   ENST00000524928 ENST00000409444 ENST00000409883 ENST00000359933 ENST00000326382 ENST00000413003 ENST00000388802 ENST00000286719 ENST00000374695 ENST00000375647 ENST00000404138 ENST00000319190 ENST00000394850 ENST00000441627 ENST00000265346 ENST00000433667 ENST00000309315 ENST00000413916 ENST00000574979 ENST00000571189 ENST00000576962

Gene Information

Description Not Available

Gene Symbol:   SRRM3
Gene Name:   serine/arginine repetitive matrix 3
Aliases:   FLJ35271, FLJ37078
RefSeq:   NC_000007.13 NT_007933.15
Ensembl:   ENSG00000177679
Entrez:   222183
Chromosome Mapping:   7q11.23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.690000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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