PrimePCR™ Probe Assay: PNPLA2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0054516
Assay Design:   exonic
Chromosome Location:   11:825080-825229question
Amplicon Length:   120
Splice Variants Targeted:   ENST00000336615

Gene Information

This gene encodes an enzyme which catalyzes the first step in the hydrolysis of triglycerides in adipose tissue. Mutations in this gene are associated with neutral lipid storage disease with myopathy. [provided by RefSeq Jul 2010]

Gene Symbol:   PNPLA2
Gene Name:   patatin-like phospholipase domain containing 2
Aliases:   1110001C14Rik, ATGL, DKFZp667M109, PEDF-R, TTS-2.2, TTS2
RefSeq:   NC_000011.9 NG_023394.1 NT_009237.18
Ensembl:   ENSG00000177666
Entrez:   57104
UniGene:   Hs.654697
Chromosome Mapping:   11p15.5

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.660000
Efficiency 104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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