PrimePCR™ Probe Assay: GBA, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: GBA, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: GBA, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0054514
Assay Design:   exonic
Chromosome Location:   1:155204702-155204791question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000368373 ENST00000327247 ENST00000427500 ENST00000536770 ENST00000428024 ENST00000574670 ENST00000572338 ENST00000602894 ENST00000602678 ENST00000602667 ENST00000602606 ENST00000602671 ENST00000602746

Gene Information

This gene encodes a lysosomal membrane protein that cleaves the beta-glucosidic linkage of glycosylceramide an intermediate in glycolipid metabolism. Mutations in this gene cause Gaucher disease a lysosomal storage disease characterized by an accumulation of glucocerebrosides. A related pseudogene is approximately 12 kb downstream of this gene on chromosome 1. Alternative splicing results in multiple transcript variants. [provided by RefSeq Jan 2010]

Gene Symbol:   GBA
Gene Name:   glucosidase, beta, acid
Aliases:   GBA1, GCB, GLUC
RefSeq:   NC_000001.10 NT_004487.19 NW_003315906.1 NG_009783.1
Ensembl:   ENSG00000177628
Entrez:   2629
Chromosome Mapping:   1q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998600
y-intercept 35.920000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
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