This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: CETN1, Human
PrimePCR™ Template for Probe Assay: CETN1, Human
The protein encoded by this gene plays important roles in the determination of centrosome position and segregation and in the process of microtubule severing. This encoded protein is localized to the centrosome of interphase cells and redistributes to the region of the spindle poles during mitosis reflecting the dynamic behavior of the centrosome during the cell cycle. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.