This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: BOK, Human
PrimePCR™ Template for Probe Assay: BOK, Human
The protein encoded by this gene belongs to the BCL2 family members of which form homo- or heterodimers and act as anti- or proapoptotic regulators that are involved in a wide variety of cellular processes. Studies in rat show that this protein has restricted expression in reproductive tissues interacts strongly with some antiapoptotic BCL2 proteins not at all with proapoptotic BCL2 proteins and induces apoptosis in transfected cells. Thus this protein represents a proapoptotic member of the BCL2 family. [provided by RefSeq Sep 2011]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.