PrimePCR™ Probe Assay: PHLDB3, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0054448
Assay Design:   exonic
Chromosome Location:   19:44008061-44008173question
Amplicon Length:   83
Splice Variants Targeted:   ENST00000322641 ENST00000238146 ENST00000292140 ENST00000599242 ENST00000594808 ENST00000601646 ENST00000264018 ENST00000505450 ENST00000508167 ENST00000510224 ENST00000447510 ENST00000540805 ENST00000539194 ENST00000543373 ENST00000380574 ENST00000449793 ENST00000312221 ENST00000347936 ENST00000596625

Gene Information

Description Not Available

Gene Symbol:   PHLDB3
Gene Name:   pleckstrin homology-like domain, family B, member 3
Aliases:   FLJ40193, MGC87511
RefSeq:   NC_000019.9 NG_008141.1 NT_011109.16
Ensembl:   ENSG00000176531
Entrez:   653583
UniGene:   Hs.631581
Chromosome Mapping:   19q13.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 35.300000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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