PrimePCR™ Probe Assay: HSD11B2, Human

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Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0054433
Assay Design:   exonic
Chromosome Location:   16:67471230-67471365question
Amplicon Length:   106
Splice Variants Targeted:   ENST00000326152

Gene Information

There are at least two isozymes of the corticosteroid 11-beta-dehydrogenase a microsomal enzyme complex responsible for the interconversion of cortisol and cortisone. The type I isozyme has both 11-beta-dehydrogenase (cortisol to cortisone) and 11-oxoreductase (cortisone to cortisol) activities. The type II isozyme encoded by this gene has only 11-beta-dehydrogenase activity. In aldosterone-selective epithelial tissues such as the kidney the type II isozyme catalyzes the glucocorticoid cortisol to the inactive metabolite cortisone thus preventing illicit activation of the mineralocorticoid receptor. In tissues that do not express the mineralocorticoid receptor such as the placenta and testis it protects cells from the growth-inhibiting and/or pro-apoptotic effects of cortisol particularly during embryonic development. Mutations in this gene cause the syndrome of apparent mineralocorticoid excess and hypertension. [provided by RefSeq Feb 2010]

Gene Symbol:   HSD11B2
Gene Name:   hydroxysteroid (11-beta) dehydrogenase 2
Aliases:   AME, AME1, HSD11K, HSD2, SDR9C3
RefSeq:   NC_000016.9 NG_011482.1 NG_016549.1 NT_010498.15
Ensembl:   ENSG00000176387
Entrez:   3291
UniGene:   Hs.1376
Chromosome Mapping:   16q22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.940000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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